The human genome is exposed to potentially deleterious genotoxic events during every cell division cycle. This endogenous source of DNA damage results from cellular metabolism or routine errors in DNA replication and recombination. In addition, cellular and organismal exposure to exogenous genotoxic agents such as ionizing radiation, ultraviolet light, oxidative stress, and chemical mutagens, may lead to a variety of nucleotide modifications and DNA strand breaks. Several assays are available to assess the mechanisms underlying DNA damage and repair.
PAR & PARP Assays
Assays that detect Poly ADP-ribose (PAR) and PAR Polymerase (PARP).
Single Cell Gel Electrophoresis Assay/CometAssay™
A simple and effective method for evaluating DNA damage in single cells.
Superoxide Dismutase Assay Kits
A simple and fast plate-based method for assaying Superoxide Dismutase activity.